Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reaction mixture used for reverse transcription of cDNA.
| Amount | |
|---|---|
| Positive control RNA | 2 μg |
| Oligo(dT)15 | 2 μl |
| dNTP mixture (10 mM) | 5 μl |
| RNase inhibitor | 1 μl |
| M-MLV reverse transcriptase (200 U/μl) | 1 μl |
| 5X M-MLV RT buffer | 5 μl |
| RNase free dH2O | Added to make 25 μl |
