Figure 3.

Effects of depletion of Bmi-1 expression on p-AKT, Bcl-2, Bax, Beclin-1 and LC3 levels. SK-siNS and SK-siBmi1 cells were incubated for 72 h with or without 5-FU treatment. The protein expression of p-AKT, Bcl-2, Bax, Beclin-1 and LC3 was detected by western blot analysis. All assays were conducted in triplicate. siNS, negative control siRNA oligonucleotides; 5-FU, 5-fluorouracil; siBmi1, Bmi-1 targeted siRNA oligonucleotide; Bmi-1, B-cell-specific Moloney murine leukemia virus insertion site 1; LC3, light chain 3.