Table I.
Antibodies and working concentrations.
| Antibody | Species isotype | Working dilution | Source |
|---|---|---|---|
| TRα1/2 | Polyclonal rabbit IgG | 1:200 | Abcam, Cambridge, MA, USA |
| TRα1 | Polyclonal rabbit IgG | 1:1000 | AbD Serotec Oxford, UK |
| TRα2 | Monoclonal rabbit IgG1 | 1:200 | AbD Serotec, Oxford, UK |
| TRβ1/2 | Polyclonal rabbit IgG | 1:200 | Zytomed, Berlin, Germany |
| TRβ1 | Polyclonal rabbit IgG | 1:200 | Millipore, Schwalbach, Germany |
| TRβ2 | Polyclonal rabbit IgG | 1:100 | Millipore, Schwalbach, Germany |
| RXRα | Mouse monoclonal IgG | 1:150 | Perseus Proteomics Inc., Tokyo, Japan |
| PPARγ | Rabbit polyclonal IgG | 1:1000 | Abcam, Cambridge, MA, USA |
| VDR | Mouse monoclonal IgG2a | 1:100 | AbD Serotec, Oxford, UK |
TR, thyroid receptor; RXR, retinoid X receptor; PPAR, peroxisome proliferator-activated receptor; VDR, vitamin D receptor.