Table II.
Strain | Immunogold Density
|
Rubisco in Pyrenoid | ||
---|---|---|---|---|
Pyrenoid | Stroma | Cytoplasm | ||
particles mm−2 | % | |||
Wild type | 149 ± 63 | 6.2 ± 1.5 | 3.8 ± 1.7 | 80 |
18–7G | N.A. | 1.3 ± 0.9 | 1.6 ± 0.6 | N.A. |
Both cell lines were grown in moderate light (150 μmol mm−2 s−1) on acetate medium. To quantitate the number of immunogold particles in the stroma and in the pyrenoid the method described in the legend to Table I was employed. Because the pyrenoid is absent from 18–7G, the number of particles in the pyrenoid is not applicable (N.A.). Ten samples of each strain were sampled. The data shown are the averages ± sd.