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Effect of MACC1-siRNA on gemcitabine-induced apoptosis. CFPAC-1 cells were transfected with mock or MACC1-siRNA for 24 h and treated with or without gemcitabine (0.5 μg/ml) for 48 h. Total protein was obtained and samples were subjected to western blot analysis for various cell apoptosis-associated proteins (Bcl-2, caspase-3, caspase-9, PARP-1). β-actin served as a loading control. MACC1, metastasis-associated in colon cancer-1.
