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Effect of gemcitabine combined with MACC1-siRNA on the Ras-ERK signaling pathway. Whole lysates from CFPAC-1 cells treated as indicated in Patients and methods, were subjected to western blot analysis with antibodies against Ras, phosphor-ERK, ERK and Gab2. β-actin served as a loading control. MACC1, metastasis-associated in colon cancer-1.
