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. Author manuscript; available in PMC: 2012 Nov 26.
Published in final edited form as: Chem Res Toxicol. 2010 Apr 19;23(4):821–835. doi: 10.1021/tx900446r

Figure 3. Peroxynitrite-mediated oxidations: slow infusion versus bolus addition.

Figure 3

Figure 3

Figure 3

BTBE (0.3 mM) was incorporated to EYPC and DLPC liposomes (30 mM) and exposed to peroxynitrite either as a single bolus (■) or by slow infusion (○) in order to achieve final concentrations of (0.2–2 mM). Samples were analyzed for 3-nitro-BTBE, 3,3´-di-BTBE and MDA content. EYPC: (A) MDA (B) 3-nitro-BTBE (C) 3,3´-di-BTBE; DLPC: (D) 3-nitro-BTBE and 3,3´-di-BTBE (inset); (E) BTBE (0.3 mM) containing liposomes were exposed to slow infusion of peroxynitrite (1 mM) in the presence and absence of oxygen and 3-nitro-BTBE and 3,3´-di-BTBE was measured as previously.