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. 2012 May;17(5):365–386. doi: 10.1111/j.1365-2443.2012.01594.x

Figure 8.

Figure 8

Predicted catalytic residues and the N-terminal cysteine cluster in AHO-3 are essential for thermotactic plasticity. (A, B) Rescue experiment for the abnormal thermotactic plasticity of aho-3(nj15) mutants with recombinant AHO-3::EGFP containing a mutation in the predicted catalytic residues (A) or into the N-terminal cysteines (B). Recombinant proteins were expressed pan-neuronally. Thermotaxis of well-fed or starved animals cultivated at 23 °C. n ≥ 3 assays. Error bars represent SEM. Asterisks represent the comparison of starved transgenic animals with starved aho-3 mutants by Dunnett test; *P < 0.05; **P < 0.01.(C–H) Subcellular localization of AHO-3(wild-type)::EGFP (C), AHO-3(C34, 35, 38, 39S)::EGFP (E) and AHO-3(C34S)::EGFP (G) expressed under the control of the aho-3 promoter in adult aho-3(nj15) mutants. Solid arrowheads point to the localization of AHO-3(wild-type)::EGFP and AHO-3(C34S)::EGFP to sensory endings (C and G). AHO-3(C34, 35, 38, 39S)::EGFP was localized diffusely in cell bodies and not to sensory endings (open arrowhead; E). Schematic diagrams of (C), (E) and (G) are shown in (D), (F) and (H), respectively. Images are Z-stack confocal projection. A rough outline of head of animal is shown (C, E and G). Anterior is to the left. Bars represent 10 μm.