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The JEG-3 cells were treated with TGF-β1 for 48 h and their invasive capacity was subsequently detected using the Transwell invasion assay. The total number of transmigrated cells was obtained by counting the number of dyed cells with a light microscope. Exogenous TGF-β1 specifically promoted the invasive capacity of JEG-3 cells. The data are presented as the means ± SD (n=5; *P<0.05 when compared with TGF-β1 0 μg/l; #P<0.05 when compared with any other group except 0 μg/l).
