Table II.
Antibody | Dilution | Manufacturer | Antigen retrieval method | Positive control |
---|---|---|---|---|
GLUT-1 | 1:50 | Dako, Carpinteria, CA, USA | Enzyme | Erythrocytes |
PI3K | 1:100 | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Microwave | Breast carcinoma |
p-Akt | 1:50 | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Microwave | Glioblastoma multiforme |
GLUT-1, glucose transporter 1; PI3K, phosphatidylinositol 3-kinase; p-Akt, phosphorylated protein kinase B.