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. 2012 Oct 31;6(5):396–404. doi: 10.4162/nrp.2012.6.5.396

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©2012 The Korean Nutrition Society and the Korean Society of Community Nutrition

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1 — Effects of the calcium treatment on cell growth, attachment and invasion in human colon cancer cells. Percentage of viable cells after the treatment of HCT116 cells with 0.8, 1.2, and 2.0 mM calcium for 72 h (A) and HT29 cells with 0.4, 0.8, 1.6 and 2.4 mM calcium for 48 h in serum complete medium (B). Percent of attached cells on the bottom of culture plate after the treatment of HCT116 cells with 0.8, 1.2, and 2.0 mM calcium in serum complete medium (C). Percentage of invaded cells to the outer chamber containing serum complete media from the inner chamber containing serum free media after the treatment of HCT116 cells with 0.4, 0.8, 1.2, 1.6 and 2.0 mM calcium for 48 h (D). The values shown are mean ± SE. Different letters (a-d) indicate statistical difference by one-way ANOVA followed by Tukey test (P < 0.05).