Figure 4. Subcellular localization of Nectin-1 and StarD10 in MCF7-SNAI1 cells upon induction of SNAI1.
(A), Immunofluorescence and phalloidin staining realized with specific anti-Nectin-1 and anti-E-Cadherin antibodies in non-induced (+Tet) MCF7-SNAI1 cells. (B), (C), Immunofluorescence and DAPI staining realized with StarD10-, E-Cadherin- (B) or ZO-1-specific (C) antibodies in non-induced (+Tet) MCF7-SNAI1 cells. (D), (E) Immunofluorescence and DAPI staining were realized in non-induced (+Tet) and induced (− Tet, 48 h after induction) MCF7-SNAI1 cells using specific anti-SNAI1 (D,E), anti-Nectin-1 (D) or anti-Stard10 (E) antibodies. Bars, 10 μm.