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. 2012 Nov 19;2012:913523. doi: 10.1155/2012/913523

Figure 1.

Figure 1

(a) IGH@ gene structure, VDJ rearrangement, and PCR primer annealing sites. The IGH@ gene is composed of ~45 V segments, ~23 D segments, and 6 J segments. DJ rearrangement occurs first, here combining DH3 and JH5. This is followed by V-DJ rearrangement, here combining VH2 with the previously rearranged DH3-JH5. Multiple PCR primers are directed against the 3 FR regions in the V segment and a single primer to the J segment (FR4). N represents nucleotides inserted and deleted by TdT. Adapted with permission from [4]. (b) TRG@ gene structure, VJ rearrangement, and PCR primer annealing sites. The TRG@ gene is composed of 11 V segments and 5 J segments. VJ rearrangement here combines the Vγ6 and JγP2 segments. Multiple PCR primers are directed against Vγ segments and Jγ segments.