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. 2012 Nov 18;2012:850684. doi: 10.1155/2012/850684

Figure 2.

Figure 2

SABG expression (a) of serially passaged HDFs in the absence or presence of 50 and 100 μM EGCG. The quantitative result (b) showed that the HDFs at early passage (PN 5) that were not treated with EGCG did not stain blue, but the cells at late passage (PN 30 or ≥40) showed much blue staining of senescent cells. Serial passage-induced senescence in HDFs was partly prevented at 50 μM EGCG, while 100 μM EGCG treatment significantly reduced the number of senescent cells. All variables were tested in three independent cultures for each experiment, which was repeated twice independently (n = 6). The results are reported as a mean ± SD and analyzed by a Tukey HSD test. *P < 0.05 versus nontreated cells of 5 PN; # P < 0.05 versus nontreated cells of 30 or ≥40 PN.