Table 1.
Lipid classes and indexes in lipid rafts from isolated from frontal cortex of WT and APP/PS1 aged 6 and 14 months.
WT 6 months | APP 6 months | WT 14 months | APP 14 months | |
---|---|---|---|---|
Sphingomyelin (SM)1 | 3.17 ± 0.15c | 4.71 ± 0.34***c | 6.05 ± 0.81 | 7.25 ± 0.47 |
Phosphatidylcholine (PC)1 | 11.62 ± 1.37a | 12.27 ± 0.18b | 15.58 ± 0.45 | 15.85 ± 0.78 |
Cholesterol (CHO)1 | 31.88 ± 4.75 | 29.40 ± 1.01c | 25.94 ± 0.47 | 22.97 ± 0.95** |
SM/PC | 0.30 ± 0.05 | 0.48 ± 0.03** | 0.40 ± 0.06 | 0.46 ± 0.05 |
SM/CHO | 0.11 ± 0.01c | 0.16 ± 0.02c | 0.24 ± 0.03 | 0.32 ± 0.02* |
Saturated2 | 58.45 ± 0.92c | 59.95 ± 0.14c | 68.15 ± 1.46 | 71.89 ± 1.01* |
Unsaturated2 | 38.25 ± 2.20b | 31.05 ± 2.48* | 31.03 ± 1.32 | 27.45 ± 1.04* |
Monoenes2 | 20.65 ± 1.41 | 17.81 ± 1.78 | 18.46 ± 0.65 | 18.46 ± 1.05 |
LCPUFA2 | 16.72 ± 2.04a | 11.90 ± 0.63b | 12.27 ± 0.90 | 8.66 ± 0.65*** |
Total n-92 | 13.36 ± 1.22a | 10.02 ± 1.91 | 10.73 ± 0.68 | 9.80 ± 0.82 |
Total n-62 | 8.93 ± 0.57c | 7.71 ± 0.35c | 6.45 ± 0.44 | 4.92 ± 0.35** |
Total n-32 | 8.49 ± 1.38a | 5.47 ± 0.36b | 5.92 ± 0.53 | 3.78 ± 0.32*** |
saturated/unsaturated | 1.56 ± 0.13c | 1.97 ± 0.16*b | 2.23 ± 0.14 | 2.64 ± 0.15* |
saturated/n-3 | 7.75 ± 1.38b | 11.11 ± 0.76c | 12.07 ± 1.28 | 19.50 ± 1.46*** |
saturated/n-6 | 6.67 ± 0.50c | 7.80 ± 0.37c | 10.87 ± 0.92 | 14.90 ± 1.05** |
saturated/n-9 | 4.57 ± 0.54b | 6.72 ± 1.29 | 6.53 ± 0.58 | 7.63 ± 0.90 |
saturated/CHO | 1.99 ± 0.27a | 2.04 ± 0.07c | 2.63 ± 0.07 | 3.15 ± 0.11*** |
SM/unsaturated | 0.08 ± 0.01c | 0.15 ± 0.00***c | 0.20 ± 0.03 | 0.26 ± 0.02 |
SM/n-3 LCPUFA | 0.41 ± 0.06c | 0.86 ± 0.00***c | 1.08 ± 0.16 | 1.95 ± 0.14*** |
CHO/unsaturated | 0.83 ± 0.10 | 0.96 ± 0.11 | 0.84 ± 0.04 | 0.85 ± 0.07 |
CHO/LCPUFA | 1.90 ± 0.13 | 2.49 ± 0.22** | 2.17 ± 0.17 | 2.69 ± 0.15* |
Phospholipids/Cholesterol | 1.81 ± 0.35 | 1.78 ± 0.09c | 2.22 ± 0.07 | 2.61 ± 0.11** |
Unsaturation index | 105.99 ± 10.72b | 78.91 ± 5.18b | 79.32 ± 5.04 | 60.40 ± 3.18** |
Data are expressed mean ± SEM for 4–5 animals in each group of genotype and age.
Weight percent respect to total lipids from lipid raft,
weight percent respect to total fatty acids from lipid raft.
,
,
Indicate statistical differences with p = 0.1, p = 0.05 and p = 0.01 between genotypes of same age.
,
,
Indicate statistical differences with p = 0.1, p = 0.05 and p = 0.01 between ages within each genotype.