Table 2.
Cell viability of canine cruciate ligamentocytes in response to different inhibitors and SNP treatment
| Inhibitor pretreatment |
CCL cell viability (n = 8) |
CaCL cell viability (n = 8) |
|||
|---|---|---|---|---|---|
| |
|
SNP treatment |
SNP treatment |
||
| - | + | - | + | ||
| zVAD.fmk |
0 |
100 ± 6.0 |
75.5 ± 3.1 |
100 ± 5.9 |
73.6 ± 2.4 |
| |
100 μM |
92.5 ± 5.3 |
84.5 ± 4.3 |
92.2 ± 6.3 |
88.2 ± 3.6* |
| CalC |
0 |
100 ± 11.3 |
79.9 ± 5.3 |
100 ± 5.9 |
78.2 ± 7.2 |
| |
25 nM |
93.5 ± 6.9 |
73.4 ± 3.3 |
100 ± 6.0 |
68.7 ± 7.0 |
| PD98059 |
0 |
100 ± 5.6 |
75.8 ±6.4 |
100 ± 4.1 |
74.7 ± 9. 3 |
| |
10 μM |
81.2 ± 7.2* |
61.8 ± 3.1* |
78.3 ± 7.2* |
52.2 ± 9.2* |
| SB202190 |
0 |
100 ±11.3 |
73.4 ± 5.4 |
100 ± 6.0 |
74.2 ± 7.7 |
| |
10 μM |
74.2 ±2.1* |
65.9 ± 4.5 |
75.2 ± 5.8* |
79.8 ± 2.4 |
| Genistein |
0 |
100 ± 6.0 |
69.6 ± 6.1 |
100 ± 5.8 |
72.3 ± 5.7 |
| |
50 μM |
101 ± 6.4 |
97.4 ±9.3** |
106.1 ±6.3 |
101.9 ± 2.5** |
| NS-398 |
0 |
100 ± 5.4 |
64.9 ± 3.6 |
100 ± 5.4 |
63.2 ± 4.1 |
| |
50 μM |
89.5 ± 17.7 |
78.1 ± 3.9* |
88.5 ± 4.7* |
88.8 ± 2.5** |
| |
100 μM |
74.7 ± 14.4 |
75.3 ± 4.2* |
81.8 ± 12.1 |
92.4 ± 2.7** |
| SN-50 |
0 |
100 ± 8.3 |
74.6 ± 3.8 |
100 ± 8.4 |
81.2 ± 2.4 |
| |
50 μM |
97.3 ± 8.6 |
64.4 ± 12.6 |
101.4 ± 4.5 |
67.3 ± 2.7* |
| PDTC |
0 |
100 ± 8.3 |
74.6 ± 3.8 |
100 ± 8.4 |
81.2 ± 2.4 |
| |
10 μM |
42.7 ± 7.7** |
5.5 ± 1.9** |
43.8 ± 2.3** |
2.1 ± 1.7** |
| Uric acid |
0 |
100 ± 9.2 |
73.5 ± 5.4 |
100 ± 5.6 |
78.2 ± 2.4 |
| |
0.5 mM |
103.6 ± 9.5 |
102.7 ± 12.1* |
101.9 ± 6.2 |
94.6 ± 5.9* |
| PTIO |
0 |
100 ± 6.2 |
64.9 ± 2.6 |
100 ± 9.3 |
61.2 ± 4.6 |
| |
5 μM |
87.9 ± 7.3 |
80.9 ± 1.5* |
96.2 ± 9.6 |
73.8 ± 3.2* |
| Taxifolin |
0 |
100 ± 3.7 |
76.2 ± 4.2 |
100 ± 5.6 |
74.3 ± 7.7 |
| 100 μM | 91.6 ± 11.5 | 84.8 ± 10.6 | 101.3 ± 4.1 | 97.3 ± 6.8* | |
Values correspond to the mean ± SD, calculated by using the formula from M&M section of three separate experiments of n different cell donors, each performed in triplicates. Cruciate ligamentocytes were preincubated with the indicated concentrations of the inhibitors for 2 h. SNP or none SNP were then added to the cultures and allowed to incubate for an additional 18-h period. SNP concentrations used were 0.2 to 0.25 mM. Cell viability was assayed by MTT assay. P-values indicate difference within the same type of cell and SNP treatment versus absent inhibitor: *, P < 0.05; **, P < 0.01.