Figure 2. The T400 mutation reduces potassium conduction.
(A) HCN2 (black) and T400 (gray) current traces elicited at – 150 mV for 2 s, from a holding potential of −35 mV measured in symmetrical (135 mM) potassium-only (top) or sodium-only (bottom) solutions. (B) Bar graph comparing current densities (pA/pF) of the HCN2 (black bar) and T400 (white bar) channels measured in potassium-only or sodium-only solutions. The numbers in parentheses represent the number of cells and the asterisk denotes a significant difference between HCN2 and T400 (t-test, p<0.05).