Figure 4.

Plk3 mRNA and protein expression in selected human melanoma cell lines and tissue samples. A. Real-time PCR was performed on RNA samples from normal melanocytes and selected human melanoma cell lines with DNA primers for Plk3. B. Western blot was performed on normal melanocytes and selected human melanoma cell lines with Plk3 and β-actin antibodies. C. Immunohistochemistry was performed on paraffin sections of human melanoma specimens and corresponding normal skin tissues using the Plk3 antibody.