Effect of the xyloglucan oligomers XXLGol (a) and
XXFGol (b) and of 2′-fucosyl-lactose (c) on the straight growth of
wheat coleoptiles (A) and on the elongation promoted by 2,4-D (B). Two
independent experiments, each conducted from 15 to 20 coleoptile
segments treated or not with 2,4-D (1 μm) for 62 h
were monitored and the mean increments of length (ΔL) were
plotted. In A each value of a curve is the average of 30 to 40
experiments, and vertical bars represent ses. Data are
presented as ΔL versus log sugar concentration; ΔL is the
additional increase in length between 10 and 20 h. The absolute
increase in length of controls (sets in the absence of 2,4-D or
oligosaccharides) exhibited the mean value of 3.87 mm. This value was
the reference (ΔL = 0). In B data are presented as mean
percentages of inhibition or activation of 2,4-D-stimulated growth,
calculated as percentages of activation or inhibition =
L(2,4-D + oligosaccharide) −
(L[2,4-D])/(L[2,4-D])
− L(control) × 100%, where
L(2,4-D) is the mean final length of
segments treated with 2,4-D, L(control) is
the mean final length of segments incubated without 2,4-D, and
L(2,4-D + oligosaccharide) is the mean final
length of segments treated with 2,4-D plus the oligosaccharide. Plotted
data represent the means ± se.