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. 1998 Mar;116(3):1013–1021. doi: 10.1104/pp.116.3.1013

Figure 5.

Figure 5

Effects of XXLGol (a), XXFGol (b), XXXGol (d) and the trisaccharide methyl α-l-Fuc-(1→2)-β-d-Gal-(1→2)-β-d-Xyl (e) on GA3-stimulated α-l-fucosidase in R. fruticosus protoplasts. The results are expressed as the R value in A and B and as the mean percentage of activation of GA3-stimulated response in C. Protoplasts (2 × 106) in 25 mL of buffer were incubated for 15 min with sugar up to 100 nm in the presence of 10 nm GA3. Each curve was obtained by least-squares regression of data from three to four replications carried out from three independent inducer sets. R is reported as the rate of α-1-fucosidase activity of treated protoplasts over controls. The mean percentage of activation is calculated as = R(GA3 + oligosaccharide) − (R[GA3])/(R[GA3]) − R(control) × 100, where R(GA3 + oligosaccharide) and R(GA3) are R values in protoplasts incubated with sugar and GA3 and with GA3, respectively, and R(control) is the R value in protoplasts suspended in buffer without the addition of 2,4-D or oligosaccharides.