Table 1.
Substrate specificity of UGT79B1 from Arabidopsis thaliana
| Sugar acceptora | Relative activity (%) |
|---|---|
| Cyanidin 3-O-glucoside | 100.0 ± 27.5 |
| Cyanidin 3-O-rhamnoside | ND |
| Cyanidin 3-O-rhamnosyl(1→6)glucoside | 48.1 ± 10.7 |
| Cyanidin 3,5-O-diglucoside | ND |
| Cyanidin 3-O-(6′′-O-p-coumaroylglucoside)-5-O-glucoside | ND |
| Cyanidin 3-O-(6′′-O-p-coumaroylglucoside)-5-O-(6′-O-malonylglucoside) | ND |
| Delphinidin 3-O-glucoside | 28.3 ± 0.8 |
| Pelargonidin 3-O-glucoside | 48.1 ± 8.8 |
| Kaempferol 3-O-glucoside | 265.4 ± 34.0 |
| Kaempferol 3-O-rhanoside | ND |
| Kaempferol 3-O-rhamnosyl(1→6)glucoside | 47.3 ± 2.0 |
| Kaempferol 3-O-glucoside-7-O-rhamnoside | ND |
| Quercetin 3-O-glucoside | 121.6 ± 16.7 |
| Sugar donorb | Relative activity (%) |
|---|---|
| UDP-xylose | 100.0 ± 27.5 |
| UDP-arabinose | ND |
| UDP-galactose | ND |
| UDP-glucose | ND |
| UDP-glucuronic acid | ND |
| UDP-rhamnose | ND |
ND, not detected.
a
The reactions were performed with UDP-xylose as the sugar donor.
b
The reactions were performed with cyanidin 3-O-glucoside as the sugar acceptor.