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. 2012 Jul 6;1:21. doi: 10.4103/2277-9175.98122

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Copyright: © 2012 Akbarzadeh-Sharbaf.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(a) Restriction digestion of pSLC1, 2, 3 and 4 constructs. Lanes 2, 3 and 4 pSLC1 construct; lanes 5, 6 and 7 pSLC2 construct; lanes 8, 9 and 10 pSLC3 construct and lanes 11, 12 and 13 pSLC4 construct that all of them were digested with 1. NheI, 2. NheI and BsiWI, 3. NheI and PvuI respectively. Lane 1: 1Kbp DNA ladder (Bioron, Australia) and lane 14: 100 bp DNA ladder (BioLabs, USA). (b) Restriction digestion of pSLO1, 2, 3 and 7 constructs. Lanes 2, 3 and 4 pSLO1 construct; lanes 5, 6 and 7 pSLO2 construct; lanes 8, 9 and 10 pSLO3 construct and lanes 11, 12 and 13 pSLO7 construct that all of them were digested with 1. NheI, 2. NheI and BsiWI, 3. NheI and PvuI respectively. Lane 1: 1Kbp DNA ladder (Bioron) and lane 14: 100 bp DNA ladder (BioLabs)