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(a) Explant culture of calvarial bone, cells start to isolate from bone segment (dark mass), (b) Native osteoblasts, one passage after isolation, (c) ADSCs. MSCs from third passage with spindle-shaped morphology, (d) Von Kossa staining of differentiated osteoblasts (day 14 of differentiation). Black nodules show deposition of mineralized extracellular matrix
