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Effect of phosphate on the expression of the Arabidopsis AK/HSD gene. Arabidopsis seedlings were incubated in the absence (−) or in the presence (+) of various combinations of phosphate, Suc, and mannitol, as described at the top. Northern-blot analysis using AK/HSD as a probe (top) and methylene blue staining of the 18S and 28S rRNA bands (bottom) were performed as described in Figure 2.
