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. 2012 Sep 20;1(11):1161–1168. doi: 10.1242/bio.20122824

Fig. 4. Rap2 regulates internalization of the extended conformation of LFA-1.

Fig. 4.

MAbs specific for pan-LFA-1 (38), high affinity (24) and extended (KIM127) LFA-1 used to immunoprecipitate (A) total LFA-1 and (B) internalized LFA-1 from T cells. (A,B) Left: typical western blot experiment. Right: mean ± s.d. of n = 4 experiments. (C) Internalization of extended but not high affinity conformations of LFA-1 are increased following Rap2 siRNA knockdown compared with control siRNA and WT treatment of T cells. Left: typical western blot experiment. Right: mean ± s.d. of n = 3 experiments. (D) Confocal microscopy image showing overlap of Rap2 and KIM127-expressing LFA-1 concentrated in the lamella with some lamellipodial distribution in the direction of T lymphoblast migration indicated by an arrow. Scale bar = 5 µm.