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. 2012 Aug 21;1(11):1102–1110. doi: 10.1242/bio.20122386

Fig. 4. Limb hairy2 expression requires cooperative AER/FGF and ZPA/SHH signaling.

Fig. 4.

(A) hairy2 expression obtained upon implantation of FGF8-beads in ZPA-ablated limbs. Implantation of FGF8-bead immediately after ZPA ablation induces local hairy2 expression (Ai,ii, arrow), but fails to do so after 6 hours of ZPA ablation (Aiii,iv). (Bi,ii) hairy2 expression observed after co-implantation of FGF8 and SHH beads in the AND. Only the previously observed SHH-mediated expansion of hairy2 expression is obtained. FGF beads implanted following 6 hours of SHH treatment are capable of inducing ectopic hairy2 expression around the bead (Biv, arrows). (Ci,ii) SHH-bead is unable to induce hairy2 upon AER ablation. SHH- and FGF8-beads are delimited by red and white dashed lines, respectively. Dorsal view; anterior to the top.