Fig. 5. Incidence of HeLa cells with >2 Cep135 spots associated with centrin foci as a function of time after siRNA transfection to knock down Emi1 during S phase.

The experimental protocol is outlined in Fig. 1A with the exception that fixation times ranged from 24 to 72 hours after transfection. Each data-point is the mean of three independent experiments (>200 cells counted for each time-point of each experiment) and the error bars are one standard deviation.