Fig. 6. Multiple centrosomes can bind to the same nucleus.

A GFP-tubulin labeled mononucleated kif9 null cell containing five centrosomes. At time −6:30, all five centrosomes (arrowheads) are separate from the nucleus (N). At −0:50, two centrosomes are adjacent to the nucleus as the cell begins to enter mitosis. At 0:00, the interphase MT arrays have disassembled, and two centrosomes (arrows) remain adjacent to the same nucleus. These centrosomes integrate into the nucleus and duplicate by the 1:40 frame; the three cytosolic centrosomes duplicate and separate just prior to the 9:10 frame. This cell continues through division, forming two unequal spindles that segregate chromosomes, and ultimately cleaves into three parts. Time, min:sec, bar, 5 µm.