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. 2011 Nov 3;1(2):82–91. doi: 10.1242/bio.2011018

Fig. 5. KLHL18 interacts with CUL3.

Fig. 5.

(A) Schematic figure of wild type and mutant of KLHL18. (B) FLAG-tagged KLHL18 expressing plasmids were transfected to HeLa cells and treated with 100 ng/mL Nocodazole for 12 hours before lysis. FLAG KLHL18 and endogenous CUL3 interaction was examined by immunoprecipitation (IP) and WB with indicated antibodies. (C) Wild type and mutant KLHL18 and CUL3 expressing plasmids were transfected to HeLa cells. Interactions were examined by IP-WB analysis with indicated antibodies. (D) Endogenous CUL3 and KLHL18 interaction in U2OS cells was examined by IP-WB with indicated antibodies