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. 2011 Nov 3;1(2):82–91. doi: 10.1242/bio.2011018

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© 2011. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Fig. 7. — (A) U2OS cells treated with 100 ng/mL Nocodazole for 12 hours were lysed and examined by IP-WB with indicated antibodies. (B) CSF-arrested Xenopus egg extracts were examined by IP-WB analysis with indicated antibodies. Asterisk shows non-specific band. (C) FLAG-tagged KLHL18 and Myc-tagged Aurora-A (Myc-Aur-A) expressing plasmids were co-transfected to 293T cells and their interactions were determined by IP-WB analysis with indicated antibodies. (D) FLAG-tagged KLHL18 expressing plasmid was transfected to 293T cells and the FLAG-KLHL18 and endogenous Aurora-A interaction was determined by IP-WB with indicated antibodies. (E) Bacterially expressed and purified GST fused KLHL18 or GST and 6x Histidine fused (His-) Aurora-A were incubated in vitro as indicated and their interactions were determined by GST-pull down assay. The input and GST-pull down reactions were separated by SDS-PAGE, transferred to a nitrocellulose membrane and examined by Ponceau S staining (upper panel). His-Aurora-A was examined by WB with anti-Aurora-A antibody as well (bottom panel).