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. 2012 Mar 30;1(5):473–481. doi: 10.1242/bio.2012760

Fig. 4. Overexpression of Cx26 but not Cx32 impairs LR development.

Fig. 4.

Full-length expression constructs were injected alone or in combination into single left or right blastomeres of the 4-8 cell embryos, and Pitx2c expression was analyzed in the 2-day tadpole. (A,A′) Injection scheme and cell lineage at stage 17. (A) Injections were targeted to the C2 lineage, i.e. cells which during flow stages (st. 17; A′) end up in the marginal cells of the GRP (somitic GRP; sGRP) and the adjacent lateral endodermal crest cells (LEC). (B) Summary of results. Note that right-sided injections did not alter Pitx2c expression. Note also that left-sided Cx26 overexpression prevented Pitx2c induction in the left LPM with very high statistical significance, while Cx32 had no effect on laterality, whether applied alone or in combination with Cx26. Numbers in brackets represent number of embryos analyzed. Statistical significances were calculated using Pearson's chi square test.