Fig. 6. GRP morphology and ciliation are unaffected in Cx26 morphants.

SEM-analysis of GRP ciliation and morphology in stage 17 dorsal explants of morphants injected with Cx26-MO or Co-MO into left blastomeres at the 4-cell stage. (A) Representative dorsal explant of Cx26 morphant specimen revealed normal cilia length, number of ciliated cells and cilia polarization. (B–D) Quantification of mean ciliation rates (B) and mean cilia lengths (C) of GRP cilia in a defined area (A′). (D) Cilia polarization (assessed in the same areas). Note that a not significant (n.s.) slight tendency towards fewer and shorter cilia was observed on the injected side in both Cx26 and co-MO morphants, but that differences between Cx26 morphants and co-MO injected specimens were not significant. Numbers in brackets indicate number of analyzed dorsal explants or cilia. Statistical significances were calculated using Student's t-test.