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. 2012 Jun 12;1(8):723–730. doi: 10.1242/bio.20121008

Fig. 2. Transient knockdown of FAK does not inhibit human endothelial cell proliferation.

Fig. 2.

(A) Two independent sequences targeting FAK were transfected into HUVECs by electroporation. 48 hours post transfection cell lysates were analyzed for the expression of FAK by Western blotting. (B) Cells transfected with control siRNA, FAK-si#1, or FAK-si#2 were analyzed for incorporation of BrdU in response to VEGF (50 ng/ml), FGF (25 ng/ml), or Complete Growth Medium (CGM). Data plotted are averages of triplicate replicates ± s.e.m. Experiments are representative of similar experiments conducted at least three additional times.