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. 2012 Jun 12;1(8):723–730. doi: 10.1242/bio.20121008

Fig. 3. FRNK, but not FAK knockdown, inhibits endothelial cell migration.

Fig. 3.

(A) HUVECs were infected with adenoviruses coding for GFP or GFP-FRNK or transfected with siRNA targeting either GAPDH (control) or FAK. Cells were serum starved for 16 hours. Migration was then evaluated in response to CGM, using a transwell migration assay over 4 hours. Data are presented as number of cells migrated to the bottom in triplicate determinations on a single day ± s.e.m. Data are representative of experiments performed at least three times. (B) As in (A), cells were transfected with 2 different sequences directed against FAK and then evaluated for migration to VEGF or CGM. Data are presented as number of cells migrated to the bottom in triplicate determinations on a single day ± s.e.m. Data are representative of two independent experiments.