Fig. 5. Proliferation response in FAK knockdown cells is not a consequence of PYK2 compensation.

(A) Cells transfected with siRNA targeting GAPDH or FAK were analyzed for the presence of phospho-PYK2 (Y402) or total PYK2 by western blotting 72 hours post transfection. (B) HUVECs were transfected with indicated siRNA or combination. At 24 hours cells were switched to serum-free medium for 16 hours followed by replacement with either serum-free medium (−) or complete growth medium (+). Cells were analyzed for BrdU incorporation and data are expressed as average percentage positive for incorporation from triplicate determinations. Error bars represent ± s.e.m. Identical results were seen in a separate experiment.