Fig. 6. Loss of FAK397 phosphorylation, but not FAK protein expression, is associated with impaired activation of RAC.

(A) Cells were manipulated by viral expression, chemical inhibition or shRNA mediated knockdown to manipulate FAK397 phosphorylation levels, as well as FAK expression similar to Fig. 1. These cells were then serum starved, followed by stimulation with CGM treatment for 30 minutes. Cells were probed for the presence of active Rac using a GST-PAK affinity assay. Bound Rac was detected by western blotting with anti-Rac. Equivalent input was monitored using ERK2. (B) Quantification of Rac activation following CCD-based densitometric analysis of western blots. Data are normalized to basal values for each experiment and represented as the mean ± range of two independent experiments.