Fig. 7. Dominant negative Rac interferes with HUVEC proliferation and Cyclin-Dependent Kinase Inhibitor regulation.

(A) HUVECS were infected with adenoviruses coding for GFP or RacN17 and then tested for the ability to induce DNA synthesis in response to CGM using BrdU incorporation. (B) HUVECs expressing RacN17 were analyzed for alterations in cell signaling and cell cycle control proteins by western analysis as indicated. These proteins show identical changes in response to expression of FRNK (Bryant et al., 2006).