Fig. 8. FAK expression is required for vascular morphogenesis.

(A) HUVECs co-infected with lentiviruses that coded for: control shRNA and GFP; FAK-3′UTR shRNA and GFP; or FAK-3′UTRshRNA and FAK (Rescue) were co-cultured with primary human fibroblasts for 21 days. Endothelial vascular structures were visualized by monitoring RFP expression co-expressed with shRNA. Images were photographed at a final magnification of 100×. Data from the entire time course can be found in supplementary material Fig. S2. (B) HUVECs infected with GFP were plated as admixed co-cultures in the presence or absence of 1 µM PF573228. Cells were monitored and photographed at day 12 at a final magnification of 100×.