Fig. 1. Proteomic analysis of swine choroid plexus epithelial cilia.

(A) Swine CPEC cilia detached by dibucaine hydrochloride. Crude cilia in suspension were ultracentrifuged, and the resulting pellet was fixed and immunostained for acetylated alpha tubulin (Green). Bar, 20 µm. For proteomic analysis, detached cilia were enriched further by differential centrifugation followed by equilibrium sedimentation. (B) Analysis of CPEC cilia proteomics data using the Ciliome database. A database search of molecules identified in the present study was performed. If molecules were already listed, the types of cilia the data were derived from were determined; 9+0, proteome of mouse photoreceptor sensory cilia (Liu et al., 2007); 9+2, proteome of motile cilia and flagella from human bronchial epithelium (Ostrowski et al., 2002), Chlamydomonas (Pazour et al., 2005), and trypanosome (Broadhead et al., 2006); Global, datasets were obtained by comparative genomics (Avidor-Reiss et al., 2004; Li et al., 2004), transcriptional profiling of Chlamydomonas (Stolc et al., 2005), and analysis of genes containing an x-box in C. elegans (Blacque et al., 2005; Efimenko et al., 2005); Centrosome, proteome of centrosome from human lymphoblastoma (Andersen et al., 2003) and Chlamydomonas (Keller et al., 2005). The actual counts of molecules in each category are shown in the key. (C) Gene ontology terms enriched in the CPEC ciliome. The dataset of 868 CPEC ciliome was analyzed using the DAVID server. Shown in this panel are those with more than 40 gene counts, P<0.01, and more than 2-fold enrichment values.