Fig. 6. The cholinergic receptor subunits a6 (Chrna6) and b3 (Chrnb3) are (subset) specifically expressed in mdDA neurons during development.

(A) The Chrna6 and Chrnb3 genes are organized as a tandem in the mouse genome (chromosome 8) in a tail to tail orientation. (B) In situ hybridization (ISH) experiments for Chrna6, Chrnb3 and Th (position control) on sagittal sections of E13.5 mouse brain. The expression patterns of Chrna6 and Chrnb3 are restricted to the mdDA neuronal region and appear to be restricted to a subset of mdDA neurons. (C) Combined ISH (purple) and immunohistochemistry (brown) experiment using Chrna6 and Chrnb3 probes with Th antibodies confirm the co-localization and restriction to the mdDA neuronal pool of the Chrna6 and Chrnb3 transcripts. L, lateral; M, medial.