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(A) Immunoprecipitation of Gad8.HA and wild type control cultures. A western blot probed with anti-HA antibody shows the specific Gad8.HA immunoprecipitation (left). The Brilliant blue stained NUPAGE gel used for analysis by mass spectrometry (right). Intense band indicated by arrow shows Gad8.HA immunoprecipitate. (B) Mass spectrometry data analysed by ScaffoldTM 3. The number of unweighted spectra and unique peptides for each protein are shown.
