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. 2012 Jul 30;1(9):915–921. doi: 10.1242/bio.2012877

Fig. 2. Presence of wound healing marker genes after crush injury.

Fig. 2.

(A) Immunohistochemistry for p63 (green) in 24 hpc transversal section of caudal fin. Blue represents DAPI positive nuclei. The arrow indicates an intact bony ray and the asterisk the site of crush injury. (B–M) Whole-mount in situ hybridization for mRNA of (B–E) pea3 at (B) 24 hpc (C) 24 hpa (D) 48 hpc (E) 48 hpa; (F–I) lef1 at (F) 24 hpc (G) 24 hpa (H) 48 hpc (I) 48 hpa; (J–M) junb at (J) 24 hpc (K) 24 hpa (L) 48 hpc (M) 48 hpa. Arrowheads indicate the amputation plane and asterisks indicate crush injury sites. Scale bar corresponds to 50 µm in A and 200 µm in B–M. (hpa – hours post-amputation; hpc – hours post-crush injury).