Fig. 8.

Inhibitors of pathways dysregulated by NF1 haploinsufficiency ameliorate the mean orientation (S) in NF1^+/− fibroblast. NF329 fibroblasts were treated for 2 days with inhibitors (I) of neurofibromin regulated pathways. I 1: Ras (30 μm FTI-277); I 2: Rac (50 μm NSC23766); I 3: MAPK (25 μm PD98059); I 4: mTOR (5 μm Rapamycin); I 5: ROCK (10 μm Y27632). K8: fibroblasts of an age matched control. S = <cos(2α)>: orientation of the cells on the grooves (h = 200 nm, d = 2 μm). N = 122–173 cells; p: S(NF1 untreated) ≠ S(NF1 treated). ***p < 0.001, **p < 0.01, *p < 0.05.