Fig. 3. Sumoylation enzymes in larval hematopoiesis.

(A–E) ZCL2897 (green) expression in wild type (A), Aos1^−/− (B) and PIAS^−/− (C) lymph glands and in tumors of Aos1^−/− (D) and PIAS^−/− (E) larvae. (F–H) Dome>GFP (green) in control lymph gland (F, without RNAi constructs). Reduction of Dome>GFP in lymph glands expressing Dome>Aos1^RNAi (G) and Dome>Uba2^RNAi (H). (I, J) Tumors form in animals expressing Dome>Aos1^RNAi (I) and Dome>Uba2^RNAi (J). Parental UAS-Aos1^RNAi and UAS-Uba2^RNAi classes do not produce tumors. Confocal sections (A–C,F,H,J) and fluorescent microscopy (D,E,G,I). Scale bars: 50 µm.