Fig. 2. In vitro binding of HDAC-1 to Smad7.

The C-terminal region responsible for direct interaction with Smad7 was located outside the HDAC-1 deacetylase domain. The E. coli cell-derived Flag-HDAC-1 protein and indicated variants shown in (A) were incubated with control GST and GST-Smad7 bound to glutathione-coupled beads and then collected. Proteins bound to the beads were detected by Western blotting with α-Flag (B).