Fig. 3. The ectopic expression of dpn, m3 and mγ induce overgrowth of the hinge.

(A–D′) Third instar larval wing discs stained with anti-Wg (in green in A–D, and grey in A′–D′), and anti-PH3 (in red A–D) antibodies. Longitudinal cross-sections at the positions of the white lines of panels A–D are shown in the right panels. In all panels, the expression of GFP driven by ap-Gal4 is shown in blue. (A′–D′) The hinge regions are highlighted in light red. (A,A′) A control wing disc is shown. The ectopic expression of ap-Gal4/+; UAS-dpn/+ (B,B′), ap-Gal4/+;UAS-m3/+ (C,C′), and ap-Gal4/+;UAS-mγ/+ (D,D′) results in a substantial increase in the number of mitotic cells (red) (positive for anti-phospho-histone H3) in the hinge region. See the text for quantification. Note that the over-expression of these genes did not induce Wg ectopic expression.