Table 2.
Gene | Forward | Reverse | Assay |
---|---|---|---|
miR-494 | TGAAACATACACGGGAAACCTC | Universal qPCR Primer (Invitrogen) | qPCR |
HsaU6 | CGCAAGGATGACACGCAAATTC | Universal qPCR Primer (Invitrogen) | qPCR |
mU6 | TGGCCCCTGCGCAAGGATG | Universal qPCR Primer (Invitrogen) | qPCR |
mATF3 | AAGGAACTTGCAGAGCTAAGCA | GGGTGGAAAAGGAGGATTCAGTA | qPCR |
mIL-6 | TCCAGTTGCCTTCTTGGGAC | GTGTAATTAAGCCTCCGACTTG | qPCR |
Rat IL-6 | CCACCAGGAACGAAAGTCAAC | AAGGCAACTGGCTGGAAGTCT | qPCR |
Rat ATF3 | AAGGAACATTGCAGAGCTAAGCA | GGGTGGAAAAGGAGGATTCAGTA | qPCR |
Rat IL-6 | CTCTCCGCAAGAGACTTCCAG | GCCGAGTAGACCTCATAGTGA | RT-PCR |
ATF3 | TTGGATCCATGATGCTTCAACAC | CCCAAGCTTTTACTTGTCATCGTC | RT-PCR |
IL-6 | TGCTCAAGTGCTGAGTCACT | AGACTCATGGGAAAATCCCA | ChIP |
qPCR, quantitative PCR.