Table 2.
Primers used
| Gene | Forward | Reverse | Assay |
|---|---|---|---|
| miR-494 | TGAAACATACACGGGAAACCTC | Universal qPCR Primer (Invitrogen) | qPCR |
| HsaU6 | CGCAAGGATGACACGCAAATTC | Universal qPCR Primer (Invitrogen) | qPCR |
| mU6 | TGGCCCCTGCGCAAGGATG | Universal qPCR Primer (Invitrogen) | qPCR |
| mATF3 | AAGGAACTTGCAGAGCTAAGCA | GGGTGGAAAAGGAGGATTCAGTA | qPCR |
| mIL-6 | TCCAGTTGCCTTCTTGGGAC | GTGTAATTAAGCCTCCGACTTG | qPCR |
| Rat IL-6 | CCACCAGGAACGAAAGTCAAC | AAGGCAACTGGCTGGAAGTCT | qPCR |
| Rat ATF3 | AAGGAACATTGCAGAGCTAAGCA | GGGTGGAAAAGGAGGATTCAGTA | qPCR |
| Rat IL-6 | CTCTCCGCAAGAGACTTCCAG | GCCGAGTAGACCTCATAGTGA | RT-PCR |
| ATF3 | TTGGATCCATGATGCTTCAACAC | CCCAAGCTTTTACTTGTCATCGTC | RT-PCR |
| IL-6 | TGCTCAAGTGCTGAGTCACT | AGACTCATGGGAAAATCCCA | ChIP |
qPCR, quantitative PCR.