Figure 2.

MC reconstitution of Kit^W-sh/W-sh mice limits anti-MPO renal injury and systemic autoimmune anti-MPO responses. (A–C) Structural renal injury, assessed by quantifying percentage abnormal glomeruli and glomerular fibrin deposition, was significantly attenuated in MPO-immunized Kit^W-sh/W-sh mice reconstituted with bone marrow-derived wild-type MCs (n=9–10) compared with immunized nonreconstituted Kit^W-sh/W-sh mice (n=10). (D) Functional injury assessed by the development of proteinuria and quantitated by creatinine/protein ratio was also significantly reduced by MC reconstitution. (E) Concordantly, glomerular influx of neutrophils, macrophages, and CD4+ T cells was decreased in wild-type MC-reconstituted Kit^W-sh/W-sh mice compared with Kit^W-sh/W-sh mice. (F–H) Systemic anti-MPO autoimmunity was also reduced in wild-type MC-reconstituted Kit^W-sh/W-sh mice assessed by dermal footpad MPO DTH response, MPO recall T cell proliferation, and IL-17A production compared with Kit^W-sh/W-sh mice. (I) The percentage of CD4+foxp3+ Tregs were restored after wild-type MCs had repopulated in Kit^W-sh/W-sh mice. Results are representative of two independent experiments, with error bars representing mean ± SEM. Statistical analysis is by unpaired t test. *P<0.05, **P<0.01, and ***P<0.001.