Figure 5.

MC-derived IL-10 attenuates MPO autoimmunity and protection against renal injury. (A–D) Reconstituting Kit^W-sh/W-sh mice with IL-10−/− MCs (n=10) did not modulate anti-MPO GN with increased percentage of abnormal glomeruli, glomerular fibrin deposition, and elevated urinary protein compared with wild-type IL-10+/+ MC-reconstituted Kit^W-sh/W-sh mice (n=10). (E) Accumulation of glomerular leukocytes correlated with disease severity, with increased numbers of neutrophils, macrophages, and CD4+ T cells in IL-10−/− MC-reconstituted Kit^W-sh/W-sh mice. (F–H) Systemic cell-mediated MPO-specific DTH footpad responses were enhanced in IL-10−/− MC-reconstituted Kit^W-sh/W-sh mice, resulting from increased T cell proliferation and IL-17A production compared with wild-type MC-reconstituted Kit^W-sh/W-sh mice. (I) Decrease in the proportion of CD4+foxp3+ Tregs in the LNs draining MPO immunization sites was apparent in IL-10−/− MC-reconstituted mice compared with wild-type MC-reconstituted Kit^W-sh/W-sh mice. Data representative of two independent experiments. Error bars depict mean ± SEM. *P<0.05.